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KMID : 0545120020120030483
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 3 p.483 ~ p.489
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Gα12 and Gα13 Subunits Modulate Ca^2+-Induced Histamine Release in Human Umbilical Cord Blood-Derived Mast Cells
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Ro, Jai Youl
Kim, Ji Young/Ha, Ji Hee/Lee, Chang Ho
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Abstract
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The role of Gαl2 and Gα13 in modulating the IgE receptor-mediated histamine secretion in the streptolysin-O-permeabilized human cultured mast cell was investigated. The expression of Gα12 and Gα13 proteins were regulated during human cultured mast cell differentiation, and a significant correlation was observed between the levels of expression of Gal2 and Gal3 proteins and IgE receptor-mediated histamine secretion capability in human cultured mast cells. Antibodies against Gα12 and Gα13 effectively inhibited the IgE receptor-induced histamine release, and the concentration of anti-Gα12 antibody used to inhibit histamine secretion was shown to also inhibit the IgE receptor-mediated elevation of intracellular Ca^2+. Therefore, the results suggest that Gα12 and Gα13 play roles in modulating IgE receptoractivated Ca^2+ influx, thereby regulating histamine release in cultured human mast cells. This is the first report to show that Gα12 and Gα13 are involved in the regulation of Ca^2+ mediated exocytosis in human cultured mast cells.
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